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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in lots of tumors, which results in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was identified to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a significant position in marketing cell-cycle development within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are actually described. Two additional splice variants ended up noted but detailed nucleotide sequence facts just isn't yet offered.
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Blocking peptides are peptides that bind precisely to your focus on antibody and block antibody binding. These peptide generally includes the epitope identified by the antibody.
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This guideline outlines more doable optimization of mobile and immunoassay parameters to be sure the absolute best outcomes are...
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This tutorial outlines even more attainable optimization of cellular and immunoassay parameters to make certain the best possible outcomes are...
Antibodies sure to the blocking peptide now not bind to the epitope about the goal protein. By comparing the staining through the blocked antibody compared to the antibody alone, you can see which staining is unique.
Target Data Cyclin E belongs to your really conserved cyclin family members, whose associates are characterized by a remarkable periodicity in protein abundance throughout the cell cycle. Cyclins functionality as regulators of CDK kinases. Various cyclins exhibit distinctive expression and degradation styles which lead on the temporal coordination of each mitotic occasion. Cyclin E types a complex with and capabilities as being a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S changeover. This protein accumulates at the G1-S section boundary and is degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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